Ideally this has nothing to do with Apache Settings, provided you are uploaded and accessing the file on same path (Apache can redirect URLs if certain Rewrite Rules are set and eventually you might end up accessing a different file).
If this file is not part of a framework which enables server side caching for example PHP based template engines like Smarty; it must be a browser caching problem. Try CTRL+SHIFT+DEL on your browser and clean everything. Re-open your browser and now try to access the file.