Server Internal Error 500

I have one subdomain which is in -- public_html/test/ folder. However no matter what i have changed in the php.ini (in public_html folder, in test folder, CWP Settings PHP.ini), it still can't upload file over 128MB. May i know what is the issue?

max_execution_time = 1800
max_file_uploads = 2000M
max_input_time = 600
memory_limit = 1024M
post_max_size = 2500M
upload_max_filesize = 2000M

check for client_max_body_size in the nginx vhost